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Mutants of Escherichia coli deficient in the fermentative lactate dehydrogenase

Fairoz Mat-Jan、Komal Alam、David P. Clark1989-01-01Journal of Bacteriology

Mutants of Escherichia coli deficient in the fermentative NAD-linked lactate dehydrogenase (ldh) have been isolated. These mutants showed no growth defects under anaerobic conditions unless present together with a defect in pyruvate formate lyase (pfl). Double mutants (pfl ldh) were unable to grow anaerobically on glu…

RNA and protein synthesis mechanismsRNA Research and SplicingEnzyme Structure and Function

Site-directed mutagenesis of the catalytic residues Asp-52 and Glu-35 of chicken egg white lysozyme.

Bruce A. Malcolm、Steven Rosenberg、Michael J. Corey 等 6 位作者1989-01-01Proceedings of the National Academy of Sciences

The roles of the catalytic active-site residues aspartic acid-52 and glutamic acid-35 of chicken lysozyme (EC 3.2.1.17) have been investigated by separate in vitro mutagenesis of each residue to its corresponding amide (denoted as D52N and E35Q, respectively). The mutant enzyme D52N exhibits approximately 5% of the wi…

Enzyme Structure and FunctionProtein Hydrolysis and Bioactive PeptidesEnzyme Production and Characterization

Cloning, nucleotide sequence, and expression in Escherichia coli of the gene for poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis

Terumi Saito、Kiichi Suzuki、Jun Yamamoto 等 10 位作者1989-01-01Journal of Bacteriology

The extracellular poly(3-hydroxybutyrate) depolymerase gene from Alcaligenes faecalis T1 was cloned into Escherichia coli DH1 by using the plasmid pUC8. An A. faecalis T1 genomic library was prepared in E. coli from a partial Sau3AI digest and screened with antibody against the depolymerase. Of the 29 antibody-positiv…

biodegradable polymer synthesis and propertiesMicrobial Metabolic Engineering and BioproductionBiopolymer Synthesis and Applications

Low-molecular-weight materials on corona-treated polypropylene

Mark Strobel、Christopher Dunatov、Joan M. Strobel 等 6 位作者1989-01-01Journal of Adhesion Science and Technology

—ESCA, wettability measurements, SEM, weight-loss determinations, and an ink adhesion test were used to characterize low-molecular-weight oxidized materials (LMWOM) formed during the corona-discharge treatment of polypropylene film. Water-soluble LMWOM is readily formed by scission processes occurring during corona tr…

Surface Modification and SuperhydrophobicityAdvanced Sensor and Energy Harvesting MaterialsHigh voltage insulation and dielectric phenomena

The structure of aconitase

A.H. Robbins、C.D. Stout1989-01-01Proteins Structure Function and Bioinformatics

The crystal structure of the 80,000 Da Fe-S enzyme aconitase has been solved and refined at 2.1 A resolution. The protein contains four domains; the first three from the N-terminus are closely associated around the [3Fe-4S] cluster with all three cysteine ligands to the cluster being provided by the third domain. Asso…

Iron Metabolism and DisordersEnzyme Structure and FunctionPorphyrin Metabolism and Disorders

Oxygen plasma etching for resist stripping and multilayer lithography

Mark A. Hartney、Dennis W. Hess、David S. Soane1989-01-01Journal of Vacuum Science & Technology B Microelectronics Processing and Phenomena

Oxygen-based plasmas commonly used in resist stripping and multilayer resist patterning are contrasted to highlight the differences involved in these applications. Mechanisms for polymer etching are reviewed, with particular emphasis on silicon-containing resists proposed for bilayer lithography. While silicon-contain…

Metal and Thin Film MechanicsAdvancements in Photolithography TechniquesCopper Interconnects and Reliability

Pattern Formation in Liquid Crystals

Ágnes Buka1989-01-01Physica Scripta

What does viscous fingering have in common with crystal growth? It is not only the visual similarity of the patterns the two seemingly unrelated phenomena can generate under certain circumstances, but also the same underlying physics which controls both growth processes. The common behaviour - diffusion limited growth…

Liquid Crystal Research AdvancementsTheoretical and Computational PhysicsSolidification and crystal growth phenomena

Plenary Lecture. The blue phases. A review of experiments

P. P. Crooker1989-01-01Liquid Crystals

We present a review of the experimental aspects of research on the blue phases of chiral liquid crystals. First a general description of blue phases is given and their relation to other important condensed matter problems is mentioned. Experimental proof for the existence of blue phases is followed by a description of…

Liquid Crystal Research AdvancementsSpectroscopy and Quantum Chemical StudiesMolecular spectroscopy and chirality

Localized states in glasses

Yu. M. Galperin、В. Г. Карпов、V. I. Kozub1989-01-01Advances In Physics

The properties of amorphous materials determined by the presence of localized atomic and electronic states are reviewed. Experimental data on atomic dynamics in glasses are described. The two-level-system (TLS) model is presented, and the limits of its applicability analysed. The concept of soft atomic potentials, all…

Material Dynamics and PropertiesGlass properties and applicationsPhase-change materials and chalcogenides

Stable Decagonal Al–Co–Ni and Al–Co–Cu Quasicrystals

A.‐P. Tsai、Akihisa Inoue、Tsuyoshi Masumoto1989-01-01Materials Transactions JIM

A stable decagonal single phase in conventionally solidified Al–Co–Ni and Al–Co–Cu alloys was found to form in each composition range of 15 to 20 at% Co and 10 to 15%Ni or 15 to 20%Cu. Coexisting decagonal and crystalline phases were also formed in conventionally solidified Al70Rh15Ni15 and Al65Rh20Cu15 alloys. The st…

Quasicrystal Structures and PropertiesX-ray Diffraction in CrystallographyMineralogy and Gemology Studies

Inorganic phosphate materials

孝文 金沢1989-01-01Medical Entomology and Zoology

1. General Background of Phosphate Materials (T. Kanazawa). Phosphate Science. History of industrial phosphate materials manufacture. Characteristics of inorganic phosphate materials. Utilization of phosphates as industrial materials. Some problems in phosphate materials research. Phosphate rock - source of phosphates…

Glass properties and applicationsPigment Synthesis and PropertiesMaterial Science and Thermodynamics

A liquid crystal microlens obtained with a non-uniform electric field

Toshiaki Nose、Susumu Sato1989-01-01Liquid Crystals

A homogeneously aligned nematic liquid crystal cell with a hole-patterned electrode and with an indium-tin oxide (ITO-) coated counter-electrode has been prepared. A non-uniform electric field can be produced by the asymmetrical electrode structure. The liquid crystal director can be reoriented by applying a voltage a…

Photonic and Optical DevicesLiquid Crystal Research AdvancementsAdvanced Optical Imaging Technologies

Site-specific Oxidative DNA Damage at Polyguanosines Produced by Copper Plus Hydrogen Peroxide

J L Sagripant、Kenneth H. Kraemer1989-01-01Journal of Biological Chemistry

Oxidative DNA damage has been implicated in diverse biological processes including mutagenesis, carcinogenesis, aging, radiation effects, and chemotherapy. We examined the in vitro effect of low concentrations of Cu(II) or H2O2 alone and in combination on supercoiled plasmid DNA. As much as 10(-2) M Cu(II) or 10(-2) M…

Metal complexes synthesis and propertiesbiodegradable polymer synthesis and propertiesSynthesis and properties of polymers

The importance of PHB-synthase substrate specificity in polyhydroxyalkanoate synthesis byAlcaligenes eutrophus

Geoffrey W. Haywood、Alistair J. Anderson、E. A. Dawes1989-01-01FEMS Microbiology Letters

Alcaligenes eutrophus can accumulate poly-3-hydroxybutyrate (PHB) or polyhydroxyalkanoate (PHA) containing only 3-hydroxybutyrate (HB) and 3-hydroxyvalerate (HV) units. Granule-associated PHB-synthase was active with d(−)-3-hydroxybutyryl-CoA and d(−)-3-hydroxyvaleryl-CoA of the range of d(−)- and l(+)-3-hydroxyacyl-C…

Enzyme Catalysis and Immobilizationbiodegradable polymer synthesis and propertiesMicrobial Metabolic Engineering and Bioproduction

Linear viscoelastic behavior of dense hard-sphere dispersions

J. C. van der Werff、C. G. de Kruif、C. Blom 等 4 位作者1989-01-01Physical Review A

The complex shear viscosity of sterically stabilized colloidal dispersions of different-sized silica particles (radius a=28--76 nm) was measured with torsion resonators and a nickel-tube resonator between 80 Hz and 200 kHz. The volume fraction of the samples was varied from 0.10 to 0.60. In the intermediate-frequency…

Microfluidic and Capillary Electrophoresis ApplicationsMaterial Dynamics and PropertiesMechanical and Optical Resonators

Quantitative Analysis of Brittle Fracture Surfaces Using Fractal Geometry

John J. Mecholsky、D. E. Passoja、K. S. Feinberg‐Ringel1989-01-01Journal of the American Ceramic Society

Fractal geometry is a non‐Euclidean geometry which has been developed to analyze irregular or fractional shapes. In this paper, fracture in ceramic materials is analyzed as a fractal process. This means that fracture is viewed as a self‐similar process. We have examined the fracture surfaces of six different alumina m…

Advanced ceramic materials synthesisComposite Material MechanicsAdvanced materials and composites

Mechanical properties at the gel point of a crystallizing poly(vinyl chloride) solution

K. Te Nijenhuis、H. Henning Winter1989-01-01Macromolecules

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMechanical properties at the gel point of a crystallizing poly(vinyl chloride) solutionKlaas Te Nijenhuis and H. Henning WinterCite this: Macromolecules 1989, 22, 1, 411–414Publication Date (Print):January 1, 1989Publication History Published online1 May 2002Published inissu…

Polymer Science and PVC

Sonochemistry - The Use of Ultrasonic Waves in Synthetic Organic Chemistry

Cathy Einhorn、Jacques Einhorn、Jean‐Louis Luche1989-01-01Synthesis

All articles of this category The recent evolution of sonochemistry has led to important improvement of various synthetic processes. The generally accepted theoretical interpretation is based on the phenomenon of cavitation, i.e. the creation of bubbles in a liquid medium, which collapse with the liberation of a consi…

Ultrasound and Cavitation PhenomenaChemical Reactions and Isotopes