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Production of Recombinant Human Type I Procollagen Trimers Using a Four-gene Expression System in the Yeast Saccharomyces cerevisiae

P. David Toman、George Chisholm、Hugh McMullin 等 12 位作者2000-07-01Journal of Biological Chemistry

The expression of stable recombinant human collagen requires an expression system capable of post-translational modifications and assembly of the procollagen polypeptides. Two genes were expressed in the yeast Saccharomyces cerevisiae to produce both propeptide chains that constitute human type I procollagen. Two addi…

Protein Hydrolysis and Bioactive PeptidesEnzyme Production and CharacterizationCollagen: Extraction and Characterization

Low-k Si–O–C–H composite films prepared by plasma-enhanced chemical vapor deposition using bis-trimethylsilylmethane precursor

Yoon-Hae Kim、Seok-Kiu Lee、Hyeong Joon Kim2000-07-01Journal of Vacuum Science & Technology A Vacuum Surfaces and Films

Low-k Si–O–C–H composite films were prepared using bis-trimethylsilylmethane as a precursor and oxygen in a rf plasma reactor. The growth rate of the Si–O–C–H composite film followed a second-order exponential decay function. This behavior could be explained by the formation of nanosized voids due to Si–CH3 and OH-rel…

Metal and Thin Film MechanicsSemiconductor materials and devicesCopper Interconnects and Reliability

Characterization of Hole Transport in a New Class of Spiro-Linked Oligotriphenylamine Compounds

U. Bach、K. De Cloedt、Hubert Spreitzer 等 4 位作者2000-07-01Advanced Materials

Spiro-containing organic charge transport materials such as the one shown in the Figure are investigated by these authors. Based on detailed temperature and field-dependent mobility studies it is shown that high mobility is preserved upon dimerization via the spiro center. Exceptional thermal stability of the amorphou…

Organic Electronics and PhotovoltaicsConducting polymers and applicationsSynthesis and properties of polymers

The High Resolution Crystal Structure of Yeast Hexokinase PII with the Correct Primary Sequence Provides New Insights into Its Mechanism of Action

Paula R. Kuser、Sandra Krauchenco、O. A. C. Antunes 等 4 位作者2000-07-01Journal of Biological Chemistry

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose 6-phosphate and ADP. Two yeast hexokinase isozymes are known, namely PI and PII. The crystal structure of yeast hexokinase PII from Saccharomyces cerevisiae without substrate or c…

Enzyme Structure and FunctionAmino Acid Enzymes and MetabolismBiochemical and Molecular Research

Enhancement of the Viscosity of Carbon Dioxide Using Styrene/Fluoroacrylate Copolymers

Zhihua Huang、Chunmei Shi、Jianhang Xu 等 6 位作者2000-07-01Macromolecules

Copolymers of a fluorinated acrylate and styrene were synthesized to enhance the viscosity of liquid carbon dioxide. The phase behavior of mixtures of these polymers with carbon dioxide was measured at 295 K and pressures from 6.70 to 48.28 MPa. Miscibility pressures decreased with a decrease in the styrene content an…

Phase Equilibria and ThermodynamicsCarbon dioxide utilization in catalysisPolymer Foaming and Composites

In Situ Oxygen-Atom Erosion Study of Polyhedral Oligomeric Silsesquioxane-Siloxane Copolymer

Rene I. Gonzalez、Shawn H. Phillips、Gar B. Hoflund2000-07-01Journal of Spacecraft and Rockets

Covers advancements in spacecraft and tactical and strategic missile systems, including subsystem design and application, mission design and analysis, materials and structures, developments in space sciences, space processing and manufacturing, space operations, and applications of space technologies to other fields.

Diamond and Carbon-based Materials ResearchEnergetic Materials and CombustionSilicone and Siloxane Chemistry

Polymer/Polymer Inclusion Compounds as a Novel Approach To Obtaining a PLLA/PCL Intimately Compatible Blend

Cristian C. Rusa、Alan E. Tonelli2000-07-01Macromolecules

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication to the EditorNEXTPolymer/Polymer Inclusion Compounds as a Novel Approach To Obtaining a PLLA/PCL Intimately Compatible BlendCristian C. Rusa and Alan E. TonelliView Author Information Fiber and Polymer Science Program, North Carolina State Universit…

Polymer crystallization and propertiesAdvanced Polymer Synthesis and Characterizationbiodegradable polymer synthesis and properties

Reinforcement of General-Purpose Grade Rubbers by Silica Generated In Situ

Shinzo Kohjiya、Yuko Ikeda2000-07-01Rubber Chemistry and Technology

Abstract The use of the sol—gel process on general-purpose grade rubbers is reviewed in the absence or presence of silane coupling agents. The sol—gel reactions of tetraethoxysilane (TEOS) in epoxidized natural rubber (ENR), styrene—butadiene rubber (SBR) or butadiene rubber (BR) vulcanizates produced silica generated…

Advanced Fiber Optic SensorsPolymer Nanocomposites and PropertiesPolymer composites and self-healing

An anti-reflection coating for silicon optics at terahertz frequencies

Andrew J. Gatesman、J. Waldman、Ming Ji 等 5 位作者2000-07-01IEEE Microwave and Guided Wave Letters

A method for reducing the reflections from silicon optics at terahertz frequencies has been investigated. In this study, we used thin films of parylene as an anti-reflection (AR) layer for silicon optics and show low-loss behavior well above 1 THz. Transmittance spectra are acquired on double-sided-parylene-coated, hi…

Photonic and Optical DevicesTerahertz technology and applicationsSilicon Nanostructures and Photoluminescence

Confinement of CdSe Nanoparticles Inside MCM-41

Harish Parala、Holger Winkler、Matthias Kolbe 等 7 位作者2000-07-01Advanced Materials

Control over spatial positioning of CdSe quantum dots(QDs) is a very important criterion for device fabrication. These authors utilize the ordered array of pores provided by the mesoporous material MCM-41 to achieve this. TEM of a single CdSe@MCM-41 particle (see Figure) shows that the hexagonally ordered mesostructur…

Quantum Dots Synthesis And PropertiesMesoporous Materials and CatalysisCopper-based nanomaterials and applications

Mucins and Molluscan Calcification

Frédéric Marin、Paul L. A. M. Corstjens、Béatrice de Gaulejac 等 5 位作者2000-07-01Journal of Biological Chemistry

A cDNA expression library constructed from mantle tissue mRNA of the Mediterranean fan mussel Pinna nobilis was screened with antibodies raised against the acetic acid-soluble shell matrix of the same species. This resulted in the isolation of a 2138-base pair cDNA, containing 13 tandem repeats of 93 base pairs. The d…

Bone Tissue Engineering MaterialsPaleontology and Stratigraphy of FossilsCalcium Carbonate Crystallization and Inhibition

Origin and evolution of sculptured thin films

R. Messier、Vijayakumar C. Venugopal、Paul Sunal2000-07-01Journal of Vacuum Science & Technology A Vacuum Surfaces and Films

Sculptured thin films (STFs) are a new class of engineered columnar thin films in which their shapes can resemble oblique matchsticks, chevrons, multiple zigzags, S’s, C’s, helices, and even superhelices. They can have densities as low as 10%–30% of the bulk material and can be made of virtually any material. The orig…

ZnO doping and propertiesSemiconductor materials and devicesOptical Coatings and Gratings

Nature of the first-order antiferromagnetic-ferromagnetic transition in the Ge-rich magnetocaloric compoundsGd5(SixGe1−x)4

L. Morellón、J. Blasco、P. A. Algarabel 等 4 位作者2000-07-01Physical review. B, Condensed matter

We report that the first-order antiferromagnetic to ferromagnetic transition in the Ge-rich magnetocaloric compound ${\mathrm{Gd}}_{5}{(\mathrm{S}\mathrm{i}}_{0.1}{\mathrm{Ge}}_{0.9}{)}_{4}$ is associated with a structural phase transformation from a high-temperature ${\mathrm{Gd}}_{5}{\mathrm{Ge}}_{4}$-type Pnma to a…

Magnetic and transport properties of perovskites and related materialsMultiferroics and related materialsAdvanced Condensed Matter Physics

Optical and structural properties of ZnO films deposited on GaAs by pulsed laser deposition

Yungryel Ryu、Shan Zhu、J. D. Budai 等 6 位作者2000-07-01Journal of Applied Physics

ZnO films were synthesized on GaAs substrates at different growth conditions by pulse laser deposition. High-purity (99.999%) oxygen was used as the ambient gas. The pressure of the ambient oxygen gas for ZnO film growth was varied from 20 to 50 mTorr, and the growth temperature from 300 to 450 °C. ZnO films showed ve…

ZnO doping and propertiesGaN-based semiconductor devices and materialsGa2O3 and related materials

Synthesis of polyHEMA hydrogels for using as biomaterials. Bulk and solution radical-initiated polymerization techniques

Juliana Matos Seidel、Sônia Maria Malmonge2000-07-01Materials Research

Dense and porous polyHEMA hydrogels were synthesized by bulk and solution radical-initiated polymerization techniques. The morphologic analysis (SEM) of obtained hydrogels showed an amorphous dense structure for the samples obtained by bulk polymerization and a porous structure with non connected porous as a result of…

Surface analysis of titanium dental implants with different topographies

M.H. Prado da Silva、G.A. Soares、C.N. Elias 等 7 位作者2000-07-01Materials Research

Cylindrical dental implants made of commercially pure titanium were analysed in four different surface finishes: as-machined, Al2O3 blasted with Al2O3 particles, plasma-sprayed with titanium beads and electrolytically coated with hydroxyapatite. Scanning electron microscopy (SEM) with Energy Dispersive X-ray Analysis…

Effects of the atmosphere and substrate on the crystallization of PLZT thin films

A.Z. Simões、A.H.M. Gonzalez、M.A. Zaghete 等 5 位作者2000-07-01Materials Research

Lead lanthanum zirconate titanate [PLZT (9/65/35)] thin films were prepared by dip-coating on Si (100) or Si/Ti/Pt (100) substrates using a polymeric precursor solution and annealed at 650 °C for 3 h. Perovskite phase formation of the PLZT thin films and microstructure were analysed using XRD and SEM. Effects of Si (1…

Water holding capacity of selected soluble and insoluble dietary fibre

Nazik N. Boulos、Heather Greenfield、R. B. H. Wills2000-07-01International Journal of Food Properties

A centnfugation method was developed to determine water holding capacity (WHC) of lignin, cellulose, pectin, whole locust bean gum (LBGW) and insoluble locust bean gum (LBGI) and was compared with the AACC method using cellulose, lignin and LBGI. WHC was found to be highest for LBGW>pectin>LBGI>lignin>cellulose. Solub…

Advanced Cellulose Research StudiesPolysaccharides and Plant Cell WallsPolysaccharides Composition and Applications

Rheology of F-actin solutions determined from thermally driven tracer motion

Thomas G. Mason、Thomas Gisler、Klaus Kroy 等 5 位作者2000-07-01Journal of Rheology

We report measurements of the frequency-dependent complex shear modulus of semidilute F-actin solutions based on optical observations of the thermally excited motion of monodisperse tracer microspheres. Because the tracer spheres cause incident laser light to be strongly scattered, we determine their average motion us…

Material Dynamics and PropertiesForce Microscopy Techniques and ApplicationsSpectroscopy and Quantum Chemical Studies