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Crystal Structures of Escherichia coli Glycerol Kinase Variant S58→W in Complex with Nonhydrolyzable ATP Analogues Reveal a Putative Active Conformation of the Enzyme as a Result of Domain Motion,

Cory Bystrom、Donald W. Pettigrew、Bruce P. Branchaud 等 5 位作者1999-03-01Biochemistry

Escherichia coli glycerol kinase (GK) displays "half-of-the-sites" reactivity toward ATP and allosteric regulation by fructose 1, 6-bisphosphate (FBP), which has been shown to promote dimer-tetramer assembly and to inhibit only tetramers. To probe the role of tetramer assembly, a mutation (Ser58-->Trp) was designed to…

Protein Structure and DynamicsCancer, Hypoxia, and MetabolismEnzyme Structure and Function

Study of the Crystallization of GeSbTe Films by Raman Spectroscopy

Junji Tominaga、Nobufumi Atoda1999-03-01Japanese Journal of Applied Physics

We have investigated the Raman spectra of thermally and laser annealed Ge 2 Sb 2 Te 5 films. The thermally crystallized film included few amorphous Te–Te bonds, however a large number of the Te–Te bonds remained in the laser annealed film. It was suggested that the Te–Te amorphous bonds were one of the main reasons le…

Chalcogenide Semiconductor Thin FilmsPhase-change materials and chalcogenidesThermography and Photoacoustic Techniques

Do cathedral glasses flow?—Additional remarks

Edgar Dutra Zanotto、Prabhat K. Gupta1999-03-01American Journal of Physics

We present a revised estimate of the relaxation time at room temperature for a window glass based on extrapolated isostructural viscosity data. This estimate, while several orders of magnitude less than the previous estimate of Zanotto [Am. J. Phys. 66 (5), 392–395 (1998)], supports his conclusion that window glass ca…

Material Dynamics and PropertiesUrban Heat Island Mitigation

Ultrastructure, cell wall composition, and phylogenetic position of Pseudodictyosphaerium jurisii (Chlorococcales, Chlorophyta) including a comparison with other picoplanktonic green algae

Lothar Krienitz、Hiroshi Takeda、Dominik Hepperle1999-03-01Phycologia

A pure culture of a common colonial freshwater picoplanktonic chlorophyte, Pseudodictyosphaerium jurisii, was isolated from Lake Stechlin (Brandenburg, Germany). This strain was compared with solitary picochlorophytes with respect to morphology, ultrastructure, and the biochemical composition of the cell walls. Its ph…

Aquatic Ecosystems and Phytoplankton DynamicsAlgal biology and biofuel productionDiatoms and Algae Research

A Microscopic Gas‐Sensing Model for Ethanol Sensors Based on Conductive Polymer Composites from Polypyrrole and Poly(ethylene oxide)

Bing‐Joe Hwang、Jen‐Yi Yang、Chi‐Wen Lin1999-03-01Journal of The Electrochemical Society

A microscopic gas‐sensing model for ethanol sensors based on polypyrrole‐poly(ethylene oxide) (PPy‐PEO) composite films prepared electrochemically is proposed. Based on this model, a sensing equation was developed which indicates the dependence of sensitivity of gas sensors on the site number of a monolayer, the thick…

Conducting polymers and applicationsAnalytical Chemistry and SensorsAdvanced Chemical Sensor Technologies

Silver nanoparticle aggregate formation by a photochemical method and its application to SERS analysis

Anjali Pal、Tarasankar Pal1999-03-01Journal of Raman Spectroscopy

Photochemical reduction of silver(I) to silver nanoparticles was achieved, for the first time, in aqueous Triton X-100 medium using ascorbic acid. The particles show the usual plasmon absorption band of silver at 410 nm and a new band at 650 nm. Transmission electron microscope studies show that the size of the indivi…

Gold and Silver Nanoparticles Synthesis and ApplicationsCopper-based nanomaterials and applicationsLaser-Ablation Synthesis of Nanoparticles

Geostatistical analysis of the influence of textural, mineralogical and geochemical parameters on the geotechnical behaviour of the ‘Argilas de Aveiro’ Formation (Portugal)

C. Galhano、Fernando Rocha、Celso Gomes1999-03-01Clay Minerals

Abstract Textural, mineralogical and chemical parameters of clays belonging to the Upper Cretaceous ‘Argilas de Aveiro’ formation were determined and correlated, using geostatistical analysis, with some relevant geotechnical parameters. The data obtained made it possible to subdivide the region under study into distin…

Soil Geostatistics and MappingClay minerals and soil interactionsGeochemistry and Geologic Mapping

Adhesion Energy of Receptor-Mediated Interaction Measured by Elastic Deformation

Vincent T. Moy、Yuekan Jiao、Thomas Hillmann 等 5 位作者1999-03-01Biophysical Journal

We investigated the role of receptor binding affinity in surface adhesion. A sensitive technique was developed to measure the surface energy of receptor-mediated adhesion. The experimental system involved a functionalized elastic agarose bead resting on a functionalized glass coverslip. Attractive intersurface forces…

Polymer Surface Interaction StudiesForce Microscopy Techniques and ApplicationsNanofabrication and Lithography Techniques

Thermal Shock Resistance of Ceramic Foams

Venkata Vedula、David J. Green、John R. Hellman1999-03-01Journal of the American Ceramic Society

Thermal shock behavior of a variety of open‐cell ceramic foams was evaluated using infrared heating and forced air cooling. The extent of damage after thermal shock was determined by a nondestructive, dynamic resonance technique. The damage in foams was found to be strongly dependent on cell size and weakly dependent…

Advanced ceramic materials synthesisHigh-pressure geophysics and materialsNuclear Materials and Properties

Fabrication of Nanograined Silicon Carbide by Ultrahigh‐Pressure Hot Isostatic Pressing

Yutaka Shinoda、Takayuki Nagano、Fumihiro Wakai1999-03-01Journal of the American Ceramic Society

Dense nanograined SiC ceramics were obtained by using hot isostatic pressing (HIP). The starting powder was ultrafine β‐SiC powder, which had a mean particle size of 30 nm and contained 3.5 wt% free carbon. SiC powders‐both boron‐doped and undoped‐were densified via HIP under an ultrahigh pressure of 980 MPa at a temp…

Advanced ceramic materials synthesisAluminum Alloys Composites PropertiesAdvanced materials and composites

A Physical Model for the Drying of Gelcast Ceramics

Sarbajit Ghosal、A. Emami-Naeini、Ywh‐Pyng Harn 等 5 位作者1999-03-01Journal of the American Ceramic Society

Gelcasting is a promising new technology for manufactur‐ing advanced structural ceramic components. The process involves drying of the “green” gelcast part before densifi‐cation. The physical mechanisms that control this relatively long drying process are not well understood. In this study, several controlled experime…

Advanced ceramic materials synthesisAdditive Manufacturing and 3D Printing TechnologiesRecycling and utilization of industrial and municipal waste in materials production

Alternating electrical conductivity of polyaniline

Rodrigo Fernando Bianchi、G. F. Leal Ferreira、C.M. Lepienski 等 4 位作者1999-03-01The Journal of Chemical Physics

The ac complex conductivity σ*(f) of polyaniline (PAN) films at different doping levels and different temperatures, in the 1–100 KHz frequency range, are reported. The results are typical of a disordered medium where the real component of ac conductivity is frequency independent at low frequencies, rising for higher v…

Advanced Sensor and Energy Harvesting MaterialsConducting polymers and applicationsElectrochemical Analysis and Applications

pH and solubility of aluminium in acidic forest soils: a consequence of reactions between organic acidity and aluminium alkalinity

Ulf Skyllberg1999-03-01European Journal of Soil Science

Summary Data from two Podzol O and E horizons, sampled in 1‐cm layers at 13 points within 2 m × 2 m plots, were used to test the hypothesis that the composition of hydrogen ions (H) and aluminium (Al) adsorbed to the solid‐phase soil organic matter (SOM) determines pH and Al solubility in organic‐rich acidic forest so…

Soil and Unsaturated FlowClay minerals and soil interactionsMine drainage and remediation techniques

Oxidative coupling polymerization of 4-methyltriphenylamine

Kenji Ogino、Aiko Kanegae、Ryoji Yamaguchi 等 5 位作者1999-03-01Macromolecular Rapid Communications

4-Methyltriphenylamine was oxidatively polymerized in chloroform (CL) or propylene carbonate (PC) in the presence of ferric chloride (FeCl3) at 50°C. Polymerization proceeded more rapidly and the molecular weight of the resulting polymer was higher in CL than in PC due to the higher oxidation potential of the solution…

Fuel Cells and Related MaterialsConducting polymers and applicationsCarbon dioxide utilization in catalysis

Injection Characteristics that Improve Performance of Ceramic Coated Diesel Engines

Roy Kamo、Nagesh S. Mavinahally、Lloyd Kamo 等 5 位作者1999-03-01SAE technical papers on CD-ROM/SAE technical paper series

<div class="htmlview paragraph">Thin thermal barrier ceramic coatings were applied to a standard production direct injection diesel engine. The resultant fuel economy when compared to the standard metallic engine at full load and speed (2600) was 6% better and 3.5% better at 1600 RPM. Most coated diesel engines…

Catalytic Processes in Materials ScienceBiodiesel Production and Applications

Surface Defect Sites Formed on Partially and Fully Dehydrated MgO: An EPR/ENDOR Study

Damien M. Murphy、R.D. Farley、Ian J. Purnell 等 7 位作者1999-03-01The Journal of Physical Chemistry B

EPR and ENDOR spectroscopy have been used to investigate a variety of trapped electron centers on the surface of polycrystalline MgO. The oxide was dehydrated under vacuum at different temperatures (673−1123 K) and UV irradiated under H 2 . The dehydration process results in the formation of surface anion vacancies, w…

Advanced Condensed Matter PhysicsElectronic and Structural Properties of OxidesMagnesium Oxide Properties and Applications

Rayleigh behaviour and the threshold field in ferroelectric ceramics

David A. Hall1999-03-01Ferroelectrics

The threshold field, Et, values for various barium titanate and PZT-based ceramics have been determined by examination of the εr |-E 0 and εr ∥-E 0 relationships. The results obtained for both ‘soft’ and ’hard’ PZT ceramics gave a good fit to the Rayleigh Law over a wide range of field amplitude for E 0 E t. The linea…

Ferroelectric and Piezoelectric MaterialsMultiferroics and related materialsAcoustic Wave Resonator Technologies

Identification of d-Proline Reductase fromClostridium sticklandiias a Selenoenzyme and Indications for a Catalytically Active Pyruvoyl Group Derived from a Cysteine Residue by Cleavage of a Proprotein

Ute Kabisch、Andrea Gräntzdörffer、Angelika Schierhorn 等 6 位作者1999-03-01Journal of Biological Chemistry

Highly active D-proline reductase was obtained from Clostridium sticklandii by a modified purification scheme. The cytoplasmic enzyme had a molecular mass of about 870 kDa and was composed of three subunits with molecular masses of 23, 26, and 45 kDa. The 23-kDa subunit contained a carbonyl group at its N terminus, wh…

Photosynthetic Processes and MechanismsEnzyme Structure and FunctionRedox biology and oxidative stress

Electrochemical Modification of the Passive Oxide Layer on a Ti Film Observed by In Situ Neutron Reflectometry

Z. Tun、James J. Noël、David W. Shoesmith1999-03-01Journal of The Electrochemical Society

Anodization and the effect of subsequently applying cathodic potential to a thin‐film Ti electrode in an aqueous NaCl solution have been studied with in situ neutron reflectometry. This new technique provides further insight into the processes underlying anodic oxide formation and hydrogen absorption under cathodic po…

Corrosion Behavior and InhibitionHydrogen embrittlement and corrosion behaviors in metalsAnodic Oxide Films and Nanostructures

Surface Exposure of the Methionine Side Chains of Calmodulin in Solution

Tao Yuan、Hui Ouyang、Hans J. Vogel1999-03-01Journal of Biological Chemistry

Binding of calcium to calmodulin (CaM) causes a conformational change in this ubiquitous calcium regulatory protein that allows the activation of many target proteins. Met residues make up a large portion of its hydrophobic target binding surfaces. In this work, we have studied the surface exposure of the Met residues…

Protein Structure and DynamicsEnzyme Structure and FunctionElectron Spin Resonance Studies